Fig. 3 | Cellular & Molecular Immunology

Fig. 3

From: Macrophages reprogrammed by lung cancer microparticles promote tumor development via release of IL-1β

Fig. 3

Noncoding RNAs enriched in L-MPs are potential ligands for TLR3. a RNAs isolated from Lewis cells or Lewis-MPs were extracted, and then, the RNA samples were separated by Experion RNA StdSens and HighSens Analysis Kits (Bio-Rad, Hercules, CA, USA). b The relative abundance of RNAs isolated from Lewis cells or Lewis-MPs was analyzed by Experion RNA StdSens and HighSens Analysis Kits (BIO-RAD) (upper). The RNAs were comprehensively sequenced using the HiSeq2500 system (Illumina, San Diego, CA, USA), and the sequences of 2 sets of RNA samples were compared (bottom). c Distribution of the coding and noncoding gene transcripts from the top enriched RNAs in Lewis cells or Lewis-MPs was analyzed. d The FPKM values of all noncoding RNAs in Lewis cells or Lewis-MPs were analyzed. e A heatmap of the top ten noncoding RNAs from Lewis-MPs was generated, and Lewis cells were used as a control. f The secondary structures of Vault misc RNA and U1 snRNA were downloaded from http://asia.ensembl.org/

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