Fig. 3

JAG1-mediated myeloid Notch1 signaling reduces hepatocellular apoptosis/necrosis in IR-stressed liver. a Liver apoptosis according to TUNEL staining in ischemic mouse livers (n = 4–6 mice/group). Quantification of apoptotic cells; scale bars, 10 μm. b ELISA analysis of serum TNF-α levels (n = 3−4 samples/group). c Western-blotting-assisted analysis and relative density ratios of p-ASK1, cleaved caspase-3, Bcl-2, and Bcl-xL expression. Representative of three experiments. d ELISA analysis of serum HMGB1 levels (n = 3−4 samples/group). All data represent the mean ± SD. *p < 0.05, **p < 0.01