Fig. 4
Myeloid Notch1 signaling-induced HSF1 expression activates Snail and regulates the NLRP3-mediated immune response in IR-stressed liver. Notch1M-KO mice were injected via the tail vein with bone marrow-derived macrophages (BMMs, 5 × 106 cells/mouse) transfected with lentivirus expressing HSF1 (Lv-HSF1) or GFP control (Lv-GFP) 24 h prior to ischemia. a Representative histological staining (H&E) of ischemic liver tissue (n = 4–6 mice/group) and the Suzuki histological score. Scale bars, 100 μm. b sALT levels (IU/L) (n = 4–6 samples/group). c Immunohistochemistry staining of 4-HNE+ cells in ischemic livers (n = 4–6 mice/group). Quantification of 4-HNE+ cells; scale bars, 10 μm. d Western-blotting-assisted analysis and relative density ratios of Snail, TRX1, TXNIP, NLRP3, and ASC expression. Representative of three experiments. e ELISA analysis of serum IL-1β levels (n = 3−4 samples/group). All data represent the mean ± SD. *p < 0.05, **p < 0.01
