Fig. 4

Changes in specific peripheral cell populations were maintained in VBM transplantation recipients. a Trends in the level of changes in CD4⁺, CD8⁺, and Tregs (CD4⁺CD25⁺Foxp3⁺) were similar in the high CBMT (n = 6), low CBMT (n = 6), and VBM transplantation (n = 6) groups at POD 30. b Comparison of CD4⁺, CD8⁺, and Treg (CD4⁺CD25⁺Foxp3⁺) cells after low CBMT and VBM transplantation at POD 30 and 60. c Ratio of Tregs to CD4⁺ and CD8⁺ T cells. d Foxp3⁺ cell detection in the skin of the OMC flap. e Expression of TCR Vβ on CD4⁺ T cells in the peripheral blood at POD 30. In a, b, the asterisk denotes statistical significance (p < 0.05). In c, the asterisk and hash (#) denote statistical significance compared to the syngeneic and low CBMT groups, respectively (p < 0.05). In d, the red arrows indicate Foxp3⁺ cells (brown color in the cell nucleus). The IHC results are representative of three mice per group. Scale bar: 50 µm; magnification: ×200 (above) and 400 (below). In e, the asterisks denote statistical significance compared to the low CBMT group (p < 0.05).