Fig. 5

TILs overexpressing PGC-1α show better recall capacity and differentiate into memory T cells with enhanced metabolic fitness. a Schematic representation of the experimental setup for TIL retransfer in naive hosts. CD45.2 mice were engrafted with 300,000 B16-OVA (s.c.), received 5 Gy whole body irradiation and 100,000 CD45.1-transduced OT-1 cells (i.v.), followed by OVA/CpG vaccination (s.c.). TILs were sorted 22 days post tumor engraftment, and 10,000 cells were retransferred in CD45.2 naive hosts (i.v.) and challenged with Listeria-Ova 2000 CFU (i.v.). Analysis was performed 15 days post recall. b Tumor growth. c Representative dot plots illustrating the frequencies of transferred cells. d Frequencies of transferred cells (pooled data from two experiments). e Percentage of CD44+ CD62L+ and f KLRG1− CD127+ populations in the blood, spleen, and liver. g MFI of MitoTracker Deep Red of transferred cells in spleen and h in liver. i IFNγ production of transferred T cells in the spleen and liver. c, d Gated on CD8+, and e–i gated on CD8+ CD45.1+ GFP+. Data are representative of two independent experiments and are presented as the mean ± SD (7–9 mice per group). If <100 transferred cells were detected by flow cytometry, the mouse was removed from the analysis. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001