Fig. 1

Glutamate induces MC degranulation. a MCs were treated with glutamate at the concentrations and time periods indicated, followed by preparation of cytospin slides and staining with toluidine blue. b MCs were treated with glutamate as indicated, and the amount of released β-hexosaminidase was measured. c Cell viability of the glutamate-treated MCs. Data in b and c represent the means ± SEM (n = 3). *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. Scale bars: 25 μm