Fig. 3 | Cellular & Molecular Immunology

Fig. 3

From: Oxidized mitochondrial DNA sensing by STING signaling promotes the antitumor effect of an irradiated immunogenic cancer cell vaccine

Fig. 3

Oxidative damage to mtDNA after irradiation. a EG7 cells were treated with X-ray irradiation in the presence of the ROS scavenger BHA (BHA-plus EG7). ROS production in EG7 cells was measured using the dye DCF. b 8-OHG in EG7 cells was detected by flow cytometry. c The concentration of 8-OHdG (pg) in mtDNA (μg) isolated from EG7 cells was measured with a competitive 8-OHdG EIA kit. d Immunofluorescence staining with an anti-8-OHG antibody (green), MitoTracker (red), and DAPI (cell nuclei, blue) was performed with untreated EG7 cells and apoEG7 cells; scale bar, 10 μm. e Immunofluorescence staining for 8-OHG (red) and cell nuclei (DAPI, blue) in untreated EG7 tumor sections and irradiated EG7 tumor sections was performed; scale bar, 10 µm. Data from three independent experiments were pooled and are shown as the means ± SEM. *p < 0.05 (Student’s t-test)

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