Fig. 3

Transcriptome profiling reveals that IL-32 induces the activation of PMNs. a Flow cytometry dot plot of freshly isolated PMNs from normal pregnant women. b Wright-Giemsa staining of freshly isolated PMNs and PMNs treated with or without 100 ng·mL⁻¹ IL-32β for 4 h. Scale bar = 10 μm. c Volcano plot representing DEGs in PMNs treated with 100 ng·mL⁻¹ IL-32β for 4 h versus control PMNs. Blue dots represent downregulated DEGs, and red dots represent upregulated DEGs (FDR < 0.05 and fold change ≥2). d Major enriched GO biological processes of the DEGs. e Clustering of DEGs within the “ROS biosynthetic process” term. f Major enriched KEGG pathways of the DEGs. g-k The GSEA results of “Positive regulation of cell activation” (g), “ROS biosynthetic process” (h), “Positive regulation of inflammatory response” (i), “Regulation of cytokine production involved in immune response” (j), and “Positive regulation of leukocyte cell-cell adhesion” (k).