Fig. 4

IL-32β induces the activation of PMNs. a, b Freshly isolated PMNs were treated with 100 ng·mL⁻¹ IL-32β for 2, 4, or 8 h. The mean fluorescence intensity (MFI) of DCF was measured by flow cytometry to evaluate ROS production. c Freshly isolated PMNs were treated with 0, 25, 50, or 100 ng·mL⁻¹ IL-32β for 4 h. ROS production is presented as the MFI of DCF. The expression of CD11b (d) and CD66b (e) on PMNs treated with 100 ng·mL⁻¹ IL-32β for 1, 2 or 4 h. f q-PCR analysis of genes involved in NOX in PMNs treated with 100 ng·mL⁻¹ IL-32β for 4 h. g q-PCR analysis of cytokine genes in PMNs treated with 100 ng·mL⁻¹ IL-32β for 4 h. h ELISA analysis of IL-1β and TNFα in the culture supernatants of PMNs treated with 100 ng·mL⁻¹ IL-32β for 8 h. i The apoptosis of PMNs treated with or without 100 ng·mL⁻¹ IL-32β for 8 h was analyzed by flow cytometry. j The phagocytic activity of PMNs pretreated with or without 100 ng·mL⁻¹ IL-32β for 4 h was detected using pHrodo zymosan and analyzed by flow cytometry. The presented results are from at least three independent experiments. The data in (b) and (d–g) were analyzed by an unpaired t test, the data in (c) were analyzed by two-way ANOVA with Tukey’s multiple comparisons test, and the data in (h), (j) were analyzed with a paired t test. Error bars, mean + SD. *P < 0.05; **P < 0.01; ***P < 0.001