Fig. 2
From: Hyaluronan is a natural and effective immunological adjuvant for protein-based vaccines
HA-based vaccines stimulate antigen-specific antibody responses in BALB/c mice. a Schematic representation of the standard immunization schedule (priming + two boosters). b Antigen-specific total IgG titer in sera collected on day 30 from BALB/c mice subjected to i.m. immunization with different antigens conjugated to 200 kDa HA or injected alone (standard immunization schedule; BSA, Vк3-20, OVA, n = 12; SOD, hGH, TT, RABV G, n = 6; H5N1, n = 4). c Anti-OVA total IgG and IgG subclass titers detected on day 30 in sera of BALB/c mice subjected to i.m. immunization with 10 μg of OVA alone, conjugated to HA, or emulsified with alum following the standard schedule (n = 10 mice/group; HA vs. alum: P < 0.001). The data in both (b, c) are expressed as the optical densities (ODs) at 490 nm for different serum dilutions. d Kinetics of antigen-specific total IgG and IgG subclass concentrations in sera of immunized mice over a period of 1 year (n = 6 mice/group; HA vs. Alum: P < 0.01). The data were analyzed using multiple t-tests (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)
