Fig. 7

Time course analysis of OVA distribution in LNs from immunized mice. a Representative images of lymph nodes collected at different time points after i.m. injection of 10 µg of OVA alone, mixed with alum or conjugated to HA; sections were stained with the mIHC panel described in the “Materials and methods” section, and images were acquired at ×4 magnification with a Mantra Quantitative Pathology Workstation. Only OVA⁺ cells (yellow) and nuclei (DAPI, blue) are visualized. b Time course quantification of the intensity of OVA-associated fluorescence signals expressed as counts per lymph node (counts/LN). Cumulative data from three independent experiments are presented (n = 3 mice; 6 LNs/group). Multiple t-tests were performed (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; P > 0.05 if not indicated)