Fig. 4

miR-23a/b suppress cytosolic DNA-induced cGAS-STING signaling activation. a L929 cells transfected with the indicated mimics (upper panel) or inhibitors (lower panel) were stimulated with HT-DNA (5 µg ml⁻¹) for 6 h. Then, the amounts of cGAMP in cell extracts were measured by ELISA. L929 cells transfected with the indicated mimics (b) or inhibitors (c) were stimulated with HT-DNA (5 µg ml⁻¹) for the indicated times. Then, the cell extracts were analyzed for TBK1 and IRF3 phosphorylation by SDS-PAGE or for IRF3 dimerization by native PAGE. L929 cells transfected with the indicated mimics (d) or inhibitors (e) were stimulated with HT-DNA (5 µg ml⁻¹) for 3 h, stained with an antibody against IRF3 (red) and imaged by confocal microscopy. Cells with nuclear IRF3 staining were counted as a percentage of total cells (n = 100 cells per sample). The scale bars represent 50 μm. The data are representative of three independent experiments (mean ± SD). *p < 0.05, **p < 0.01