Fig. 1
Mn2+ promotes DC maturation via cGAS-STING activation. a Heatmap of RNA-seq analysis. BMDCs were untreated or treated with MnCl2 (200 μM) or LPS (100 ng/ml) for 20 h. A heatmap was generated by calculating log2 ((treated RPKM)/(control RPKM)). b Quantitative RT-PCR analysis of the expression of the indicated gene in the WT and Sting1−⁄− BMDCs treated with MnCl2 (200 μM) or LPS (100 ng/ml) for 20 h. c Type I IFN activity in the culture medium from WT or indicated gene-deficient BMDCs treated with MnCl2 (200 μM), VACV, or SeV for 24 h. d BMDCs from the WT or indicated gene-deficient mice were treated with the indicated concentrations of MnCl2 or LPS for 20 h. CD86, CD80, and CD40 expression was analyzed by FACS. One representative experiment of at least three independent experiments is shown, and each was performed in triplicate. Error bars represent the SEM; data were analyzed by an unpaired t-test. ns not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001
