Fig. 2

Enhanced cell survival and proliferation of CD7⁺ HTLPs in the presence of TNFα in DL-4 cultures of CB or mPB HSPCs. A,B The level of apoptosis, analyzed by staining with Annexin-V and 7-AAD (early and late apoptosis correspond to Annexin-V⁺7-AAD⁻ and Annexin-V⁺7-AAD⁺ phenotypes, respectively), among CD7⁺ HTLPs (A) and CD7⁻ cells (B) after 7 days of DL-4 culture in the presence or absence of TNFα (mean ± SEM, n = 4). P values were calculated using the Mann–Whitney rank-sum test; *p ≤ 0.05. C Assessment of cell proliferation (based on CFSE dilution) from day 3 to day 7 in DL-4 cultures in the presence or absence of TNFα. CD34⁺ cells were labeled with CFSE prior to DL-4 culture. The frequencies of quiescent (G0) cells and cells in the G1 or S/G2/M phases of the cell cycle among CD7⁺ HTLPs (D) and CD7⁻ cells (E) after 7 days of DL-4 culture in the presence or absence of TNFα (mean ± SEM, n = 3). The p values were calculated by one-way RM ANOVA: *p ≤ 0.05; **p ≤ 0.01