Fig. 3 | Cellular & Molecular Immunology

Fig. 3

From: A DL-4- and TNFα-based culture system to generate high numbers of nonmodified or genetically modified immunotherapeutic human T-lymphoid progenitors

Fig. 3

Similar transcriptomic profiles of TNFα-exposed and nonexposed CD34−CD7+ late T-cell progenitors. A PCA after RNA sequencing of sorted CB or mPB HSPC-derived CD34+CD7+ and CD34−CD7+ progenitors after 7 days of DL-4 culture. Each point represents a replicate sample. B A heat map representing the gene expression profile for the cell cycle pathway among CD34−CD7+ progenitors generated from CB HSPCs. C, D GSEA of RNA-seq data obtained from CD34−CD7+ cells generated in the presence or absence of TNFα. C The 16 pathways (out of 20) with the highest normalized enrichment scores (all p < 0.01) and D their corresponding GSEA plots, showing the enrichment profile

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