Fig. 5: Antibody response to T cell-dependent antigen (NP-CGG) in iB-μMT mice.

A Schematic diagram of iB-μMT mice and μMT mice immunized with NP-CGG and from which sera were collected at the indicated time points. iB-μMT mice (n = 7) and μMT mice (n = 7) were given primary intraperitoneal immunization with 100 μg NP-CGG in alum on Day 0. Then, iB-μMT mice (n = 5) and μMT mice (n = 5) were given a secondary challenge with 50 μg NP-CGG on Day 111 after primary immunization. Sera were collected on Day 0, Day 7, Day 14, Day 21, Day 111, Day 116, Day 121, and Day 126 after primary immunization. B T cell-dependent primary antibody response in iB-μMT mice. iB-μMT mice (n = 7) and μMT mice (n = 7) were immunized with 100 μg NP-CGG in alum on Day 0. Anti-NP IgM (top), total anti-NP IgG1 (middle), and high-affinity anti-NP IgG1 (bottom) antibodies in the sera collected at the indicated time points were measured by ELISA. Each symbol represents an individual mouse, and the horizontal lines indicate the mean values. NS, not significant, *P < 0.05, **P < 0.01, and ***P < 0.001 (independent-sample Student’s t test and Mann–Whitney U test). C Production of memory antibodies in iB-μMT mice. Sera were collected from iB-μMT mice (n = 5) and μMT mice (n = 5) at the indicated time points after boost with 50 μg NP-CGG. Levels of total anti-NP IgG1 and high-affinity anti-NP IgG1 were measured by ELISA. Each symbol represents a mouse, and the horizontal lines indicate the mean values. *P < 0.05, **P < 0.01, and ***P < 0.001 (independent-sample Student’s t test and Mann–Whitney U test)