Fig. 6: Normal germinal center (GC) B, memory B, and plasma cell formation in iB-μMT mice.

A Plasma cells and antigen-specific GC B cells in the spleens of iB-μMT mice. Spleen cells were isolated from B6 and iB-μMT mice 14 days after primary intraperitoneal immunization with 100 μg NP-CGG in alum. Plasma cells (Lin⁻B220^low/−CD138⁺) and antigen-specific GC B cells (Lin⁻B220⁺CD138⁻NP⁺GL7⁺CD38⁻) were analyzed by flow cytometry. Data from one representative B6 mouse and two representative iB-μMT mice are shown. Lin⁻ was defined as Ter119⁻Mac1⁻Gr1⁻NK1.1⁻CD3⁻CD4⁻CD8⁻. B Antigen-specific class-switched IgG1⁺ memory B cells in the spleens of iB-μMT mice. Spleen cells were isolated from B6 and iB-μMT mice 21 days after primary intraperitoneal immunization with 100 μg NP-CGG in alum. Representative flow cytometry plots of antigen-specific class-switched IgG1⁺ memory B cells (Lin⁻IgD⁻IgM⁻CD138⁻B220⁺NP⁺CD38⁺IgG1⁺) from one B6 mouse and two iB-μMT mice are shown. Lin⁻ was defined as Ter119⁻Mac1⁻Gr1⁻NK1.1⁻CD3⁻CD4⁻CD8⁻. C, D Long-lived plasma cells in the bone marrow of iB-μMT mice. Long-lived plasma cells (Lin⁻IgM⁻CD138⁺CD22⁻CD19⁻MHCII⁻) in the bone marrow of B6 and iB-μMT mice were analyzed by flow cytometry. Representative plots from B6 and iB-μMT mice 21 days after priming with NP-CGG in alum (C) and 17 days after NP-CGG boost (D) are shown. Lin^- was defined as Ter119⁻Mac1⁻Gr1⁻NK1.1⁻CD3⁻CD4⁻CD8⁻