Table 1 Oligonucleotide primers and sequences
From: SARS-CoV-2 ORF10 suppresses the antiviral innate immune response by degrading MAVS through mitophagy
Target Genes | Primer Sequences (5′ to 3′) |
|---|---|
Human-IFN-α1-F | GCCATCTCTGTCCTCCAT |
Human-IFN-α1-R | AGTTTCTCCCACCCTCTC |
Human-IFN-β-F | GGACAGGATGAACTTTGACA |
Human-IFN-β-R | AGACATTAGCCAGGAGGTT |
Human-ISG15-F | TCTGAGCATCCTGGTGAG |
Human-ISG15-R | GAAGGTCAGCCAGAACAG |
Human-OAS1-F | GCTCCTACCCTGTGTGTGTGT |
Human-OAS1-R | TGGTGAGAGGACTGAGGAAGA |
Human-β-actin-F | GGAAATCGTGCGTGACAT |
Human-β-actin-R | AAGGAAGGCTGGAAGAGT |
shORF10-F | GTTTTCGCTTTTCCGTTTAC |
shORF10-R | TTAACTACATCTACTTGTGCTATG |
