Fig. 4
Mφ-specific nanotherapy against JMJD3 improves diabetic tissue repair. A Schematic of Mφ specific, GSK-J1-laden, dextran core nanoparticles. B Wound healing curve for DIO mice wounded with a 6 mm punch biopsy, and wounds were injected daily starting on Day 1 post-injury with nanoparticles containing either a selective JMJD3 inhibitor (GSK-J1; 1 mg/kg) or dextrose control. DIO mice deficient in myeloid JMJD3 production (Jmjd3f/fLyz2Cre+) were included as a control. Wound area was measured daily with ImageJ software throughout the wound healing course (N = 5/group, repeated twice). Representative wound images at ×2 magnification on Day 0 and Day 5 are shown. Wounds were harvested on Day 5, paraffin embedded, and stained with Masson’s trichrome stain (N = 5–6 mice/group). The black bar above the wound represents the entire wound distance, the arrowheads represent the epithelial tongue edges, and the asterisk (*) represents wound debris. The scale bar represents 200 μm. C Tmem173, Il1b and Tnfa expression in DIO wound Mφs (CD3-/CD19-/NK1.1-/Ly6G-/CD11b+) harvested on Day 5 from wounds treated with and without GSK-J1 nanoparticles (N = 3–4/group, pooled, repeated in triplicate). *p < 0.05, **p < 0.01. Data are presented as the mean ± SEM. Data were first analyzed for normal distribution, and if data passed the normality test, a two-tailed Student’s t test was used
