Fig. 2

IgA1 Fab profiling reveals highly unique and stable human serum and milk intra-donor IgA1 clonal repertoires, with several clones shared between serum and milk. A The overlap between the full clonal IgA1 repertoires is given as a color-coded percentage of the total IgA1 clone abundances. Donors are annotated as colored silhouettes following Fig. 1. Data for serum and milk are annotated as red and blue droplets, respectively, with three rows/columns per donor group, one for each sample collection. B Hierarchical clustering dendrogram reveals the tight connection between IgA1 clonal repertoires from the same donor. Within a single donor the samples from either serum or milk cluster most tightly with each other. Additionally, there is close clustering between the IgA1 repertoires from serum and milk within a given donor. Samples taken from different donors are completely dissimilar. C Illustrative deconvoluted IgA1 Fab mass and concentration profiles, all obtained from Donor 1. Sample collections are annotated as C1–3, and serum and milk are indicated by red and blue droplets, respectively. Each peak represents a unique Fab (based on unique retention time and mass combination) and the peak height depicts the abundance of that clone. Red and blue peaks represent clones uniquely identified in either serum or milk, respectively. The purple peaks represent clones detected in both serum and milk. On the right of the mass profiles, the pie charts depict the percentage (in concentration) of shared clones in each measured repertoire. The red and blue pie slice represents the combined relative concentrations of clones uniquely identified in either serum or milk, respectively. The purple pie slice represents the combined relative concentration of shared clones, with the number inside the chart depicting the percentage of shared clones