Fig. 3

High CD226 expression on CD8⁺ T cells is associated with better survival. A Evaluation of CD3 with immunostaining and counting for the density in the tumor center and invasion margin with QuPath. The scale bar corresponds to 1 mm. RFS of 39 patients according to CD3 density in the tumor center, as discretized as high or low according to the median. B Comparison of CD226 levels, high or low, on CD4⁺ and CD8⁺, and CD3 density in the tumor center. CD3 density was discretized into two groups, high and low, according to the median density value of the full dataset. No significant difference was observed between the groups. C RFS according to median CD226 expression on CD4⁺ T cells discretized as high or low according to the median. D OS according to median CD226 expression on CD4⁺ T cells discretized as high or low according to the median. E RFS according to median CD226 expression on CD8⁺ T cells discretized as high or low according to the median. F OS according to median CD226 expression on CD8⁺ T cells discretized as high or low according to the median. G RFS according to CD8⁺ T cells positive or not for CD226 and PD1. Groups were discretized based on median expression. H OS according to CD8⁺ T cells positive or not for CD226 and PD1. Groups were discretized based on median expression. On survival graphs, p stands for the log-rank test. OS: overall survival; RFS: relapse-free survival