Fig. 1
From: IL-6 prevents Th2 cell polarization by promoting SOCS3-dependent suppression of IL-2 signaling
IL-6 signaling during allergen sensitization suppresses allergen-specific Th2 cell-mediated immunity. A–G B6 (WT) and Il6-/- mice were i.n. treated with 100 µg low-endotoxin HDM (HDM; endotoxin content <30 EU/mg) or high-endotoxin HDM (HDMLPS; endotoxin content: 1 µg/mg, ~250 EU/mg) for 3 days. On Day 15, the mice were i.n. challenged with 100 µg HDM daily for 3 days and then analyzed on Day 21 (A). Frequencies (B, C) and numbers (D) of IL-13+IL-5+ CD4+ T cells in the lungs. Frequencies and numbers of eosinophils, neutrophils and monocytes in the lungs (E, F). HDM-specific IgE levels in the serum (G). H–L B6 mice were i.n. sensitized with HDM or HDMLPS. Some mice also received 250 µg anti-IL-6 and anti-IL-6R (i.p.). On Day 15, the mice were i.n. challenged with HDM and then analyzed on Day 21 (H). Frequencies (I, J) and numbers (K) of IL-13+ CD4+ T cells in the lungs. Numbers of lung eosinophils (L). M–Q Mice were transferred with OTII.4get cells, i.n. treated with HDM or HDMLPS + 5 µg OVA for 3 days and analyzed on Day 5. Frequencies and numbers of total (N–O) and IL-4-GFP+ (N, P, Q) OTII cells in the mLN. Data are representative of at least three independent experiments (mean±S.D., n = 4-5, two-way and one-way ANOVA). See Fig. S1
