Fig. 6

Reduced binding of ALKBH5 to the CSF3R mRNA leads to decreased G-CSFR expression in bacteria-infected neutrophils. A, B FACS analyses of the protein levels of G-CSFR on the surface of WT dHL-60 cells stimulated with E. coli (A) or LPS (B) for the indicated times (n = 6). C, D FACS analyses of total intracellular G-CSFR protein in WT dHL-60 cells stimulated with E. coli (C) or LPS (D) for the indicated times (n = 6). E, F qRT‒PCR analysis of CSF3R mRNA level in WT dHL-60 cells stimulated with E. coli (E) or LPS (F) for the indicated times (n = 5). G RIP-qPCR analysis of ALKBH5 binding to the CSF3R mRNA in WT dHL-60 cells without or with E. coli infection for 4 h (n = 4). The data were presented relative to those obtained with IgG. All data are the mean ± SEM of biologically independent samples. The data are representative of 6 independent experiments with similar results (A–D). Two-tailed unpaired Student’s t-test (A–G). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001