Fig. 2

TRIM33 deficiency disrupted the homeostasis of DC progenitors in the bone marrow. A Schematic plot indicating the progenitors and pathways involved in the development of different DC subsets. HSC hematopoietic stem cell, MPP multipotent progenitor, CMP common myeloid progenitor, CLP common lymphoid progenitor, CDP common DC progenitor. The solid lines show confirmed developmental pathways, and the dashed line indicates a developmental relationship yet to be confirmed. The figure was created with biorender.com. B–D Flow cytometry analysis of BM progenitors. BM cells from Trim33^fl/fl or Trim33^fl/fl Cre-ER^T2 mice were pregated for viability and negative staining for lineage markers (Lin^– i.e., CD2^–CD3^–CD8^–CD11b^–CD19^–B220^–TER119^–Ly6G^–). Different progenitors were then defined by various surface markers: LSKs—Lin^–Sca-1⁺CD117^hi, long-term HSCs (LT-HSCs)—Lin^–Sca-1⁺CD117^hiCD34^–FLT3^–, short-term HSCs (ST-HSCs)—Lin^–Sca-1⁺CD117^hiCD34⁺FLT3^–, MPPs—Lin^–Sca-1⁺CD117^hiCD34⁺FLT3⁺, GMPs—Lin^–Sca-1^–CD117^hiCD34⁺CD16/32^hi, CMPs—Lin^–Sca-1^–CD117^hiCD34⁺CD16/32^lo, MEPs (megakaryocyte-erythroid progenitors)—Lin^–Sca-1^–CD117^hiCD34^–CD16/32^–, CD115⁺ CDPs—Lin^–CD11c^–CD127^–CD117^intFLT3⁺CD115⁺, CD115^– CDPs—Lin^–CD11c^–CD127^–CD117^intFLT3⁺CD115^–, CLPs—Lin^–Sca-1⁺CD117⁺CD127⁺, MDPs—Lin^–CD117^hiFLT3⁺, pre-DCs—Lin^–CD172α^–MHC-II^–FLT3⁺CD11c⁺, pre-pDCs—Lin^– CD117^int/loFLT3⁺CD115^–CD127⁺Ly6D⁺SiglecH⁺, pre-cDC1s—Lin^–CD226⁺FLT3⁺CD117^int, and pre-cDC2s—Lin^–CD117^loFLT3⁺CD115⁺. E, F The numbers (means ± SEMs) of the indicated progenitors per animal. N = 3–5. Representative data from 2-3 independent experiments are shown. The data were analyzed using two-tailed Student’s t tests. Ns: P > 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001