Fig. 2
From: TL1A and IL-18 synergy promotes GM-CSF-dependent thymic granulopoiesis in mice
In vivo administration of TL1A + IL-18 results in acute thymic atrophy and increased thymic neutrophil numbers in neonatal and adult mice. A Schematic of the experimental model of TL1A + IL-18 injection in neonatal mice. Wild-type (WT) neonatal mice (P3) were IP injected with either PBS (vehicle) or TL1A [250 ng/day] + IL-18 [100 ng/day] for 4 consecutive days in a final volume of 20 µl. B Schematic of the experimental model of TL1A + IL-18 injection in adult mice (8 weeks old). WT mice were IP injected with either vehicle (PBS) or TL1A [1 µg/day] + IL-18 [750 ng/day] for 4 consecutive days in a final volume of 200 µl. C Body weights of neonates (P7) injected with either PBS (vehicle) or TL1A + IL-18 (n = 10). Data representative of one of at least five independent experiments are shown. D Pictures of thymuses from neonates (P7) injected with either PBS (vehicle) or TL1A + IL-18. E Thymic cellularity of neonates (P7) injected with either PBS (vehicle) or TL1A + IL-18. (n = 10). Data representative of one of at least five independent experiments are shown. F Quantification of neutrophil numbers in neonatal mice (P7) injected with PBS (gray) or TL1A + IL-18 (blue). Neutrophils were defined as Lin-CD11b+Ly-6G+ cells. The number of neutrophils in the complete thymus was quantified (n = 10). Data are representative of one of at least five independent experiments. G Body weights of adult model mice on Day 5 (D5) that were injected with either PBS (vehicle) or TL1A + IL-18 (n = 10). Data representative of one of at least five independent experiments are shown. H Pictures of thymuses from adults (D5) injected with either PBS (vehicle) or TL1A + IL-18. I Thymic cellularity of adult thymuses (D5) injected with either PBS (vehicle) or TL1A + IL-18 (n = 10). Data representative of one of at least five independent experiments are shown. J Quantification of the neutrophil numbers in adult mice (D5) injected with PBS (gray) or TL1A + IL-18 (blue). Neutrophils were defined as Lin-CD11b+Ly-6G+ cells. Neutrophil numbers were quantified from complete thymuses (n = 8). Data representative of one of at least five independent experiments are shown. Statistics: (C, E, G, I) Unpaired t test with Welch’s correction. E–J The error bars represent the SDs. F–J The Mann–Whitney U test was performed, as the standard deviations were different between treatment groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
