Fig. 1
From: Silencing of SIRPα enhances the antitumor efficacy of CAR-M in solid tumors
The FcγRIIa domain enhances CAR-mediated tumor phagocytosis. A Construction of HER2-targeting CAR macrophages incorporating diverse FcγR domains. B Flow cytometry histograms illustrating the lentivirus transfection efficiency in THP-1 cells. C Fluorescence-activated cell sorting (FACS) analysis of the phagocytic efficiency of the GFP+ macrophages. GFP- and CAR-modified macrophages were cocultured with mCherry+ SKOV3 cells at an effector-to-target ratio (E:T) of 1:1 for 1 h before flow cytometry analysis. D Structural diagram of CAR and CAR-shSIRPα. Flow cytometry histograms depicting CAR and CAR-shSIRPα expression levels, with GFP (E) and His-tagged recombinant HER2 (F) in sorted THP-1 cells. G Immunoblot analysis of SIRPα protein levels in untransduced (UTD), CAR, and CAR-shSIRPα macrophages. H FACS analysis of SIRPα expression levels in unstained (USD), untreated (UTD), CAR, and CAR-shSIRPα macrophages, as well as UTD, CAR, and CAR-shSIRPα macrophages cocultured with SKOV3 cells for 24 h
