Fig. 2
From: Silencing of SIRPα enhances the antitumor efficacy of CAR-M in solid tumors
CAR-shSIRPα-treated macrophages exhibit an enhanced M1-like polarization phenotype. qRT‒PCR analysis of CD80 (A), CD86 (B), and TNF-α (C) expression levels in UTD, GFP, CAR, and CAR-shSIRPα macrophages. qRT‒PCR analysis of CD80 (D), CD86 (E), and TNF-α (F) expression levels in UTD-stimulated, and SKOV3-cocultured CAR- and CAR-shSIRPα-stimulated macrophages. G–J FACS analysis of M1- and M2-like phenotypic markers in resting macrophages and macrophages cocultured with SKOV3 cells. Expression levels of CD80, CD86, and HLA-DR in resting (G) and SKOV3-cocultured (H) UTD, CAR, and CAR-shSIRPα macrophages. Expression of CD163 and CD206 in resting (I) and SKOV3-cocultured (J) UTD, CAR, and CAR-shSIRPα macrophages. ELISA analysis of IL-1β (K), INF-γ (L), and TNF-α (M) production in UTD, CAR, and CAR-shSIRPα macrophages with or without coculture with SKOV3 cells. The data are presented as the means ± s.e.m. of three technical replicates. For all panels, *P < 0.05, **P < 0.01, ***P < 0.001
