Fig. 6

Potent antitumor activity of CAR-shSIRPα macrophages in vivo. A, B Lenti-CAR and Ad-CAR macrophages were used to treat nude mice bearing peritoneal SKOV3 tumors. A The tumor burden was assessed via bioluminescence imaging (BLI), and representative images at different time points were presented. B Kaplan‒Meier curve showing the survival of the mice (n = 5 mice per group). C The tumor burden in the B16-HER2 cell subcutaneous injection model was evaluated via BLI, and representative images at different time points were shown (n = 5 mice per group). D IHC staining and TUNEL immunofluorescence staining were performed on tumor tissue sections from the B16-HER2 cell subcutaneous injection mouse model to examine the expression of Ki67, CD31, and active caspase-3, as well as apoptosis, in the tumor tissues. Scale bars represent 100 μm. E Polarization markers for M1 (CD80 and CD86) and M2 (CD163 and CD206) infiltrating macrophages were analyzed via FACS. F, G Tumor-bearing mice were euthanized four weeks after receiving caudal vein transfusions of CAR-modified macrophages in the B16-HER2 lung metastasis model. F Representative macroscopic images of lungs excised from the specified treatment groups at the end of the experiment. G Lung metastatic burden was assessed via HE staining. Scale bars represent 1 mm (n = 7 mice per group). For all panels, *P < 0.05, **P < 0.01, ***P < 0.001