Fig. 7

Clonal expansion and exhaustion during aging. A Abundance of the three most abundant clones in each mouse from the early (n = 21) and delayed aging (n = 27) groups at the end of the study. Each marker indicates one clone. Markers of a distinct shape represent data from the same mouse in each experiment in all figures. Each horizontal black bar denotes the mean of all the clones in each group. B Fractions of expanded clones in early (n = 9) and delayed aging (n = 9) mice. Expanded clones are those whose abundance is greater than a threshold defined by the lowest abundance of the most abundant clones whose aggregate abundance is at least half of all tracked clones at the pre-divergent time point. A, B Bonferroni-adjusted independent Student’s t test between groups. C Heatmap showing the fraction of lineage-biased and balanced clones that were exhausted by the end time point. The median of each group is shown. D Relative immune cell contributions of persistent, exhausted and activated clones in individual mice. Persistent clones are defined as clones that were present at both months 4 and 9 post-transplantation, as well as at the end time point. Activated clones are defined as clones that were absent at both months 4 and 9 but were present at the end time point. C, D Exhausted clones are defined as clones that were present at both months 4 and 9 post-transplantation but were not present at the end time point. **P < 0.01, N.S. not significant, WBC white blood cells