Fig. 6 | Cellular & Molecular Immunology

Fig. 6

From: Cholesterol homeostasis and lipid raft dynamics at the basis of tumor-induced immune dysfunction in chronic lymphocytic leukemia

Fig. 6

Defective FAO leads to delayed T-cell activation, but does not affect proliferation. PBMCs from HD, CLL patients and patients with genetic FAO defects (FAO Mut) were stimulated for 2 or 5 days with αCD3/αCD28 antibodies. Patients with HADHA, CPT1A, and CPT2 mutations are indicated with circles, squares, and triangles, respectively. A FAO was evaluated in CD4+ T cells at day 2 by using FAOBlue, and fold change of intensity was calculated by dividing the fluorescence intensity of the stimulated samples by that of the matched unstimulated samples in all groups. B Expression of CD25 on CD4+ T cells was measured on days 2 and 5. C Proliferation was measured at day 5 and is represented by percentage of divided cells (left) and division index (middle). Representative CTV histograms are shown (right); HD (black), CLL (gray), and FAO Mut (orange). Data are presented as mean ± SEM and differences were analyzed with two-way repeated measures ANOVA with Tukey’s/Šidák’s multiple comparison test. *** = p < 0.001; ** = p < 0.01; * = p < 0.05

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