Fig. 2
Trajectory analysis of macrophage subclusters in silicosis revealed distinct features. Differentiation trajectory of macrophages in all lung samples with points colored by a macrophage subcluster and b pseudotime. c Differentiation trajectory of each macrophage subcluster. d Differentiation trajectory of macrophages in the control (0 d), inflammation (3 d and 7 d), and fibrosis (14 d, 28 d, and 56 d) groups. e Pseudoheatmap showing the various genes involved in the differentiation process of macrophages, which were clustered into 4 clusters related to fibrosis, inflammation, antigens, and translation. f Schematic diagram of the silicosis mouse model. g mRNA expression levels of Spp1, Mmp12, Pla2g7, Il17r, and Il1rn in AM subclusters sorted from silicosis and control mouse lung tissue, n = 3 per group. h, i Proportion of IM subclusters and AM subclusters in silicosis mouse lungs analyzed by FC. n = 3 per group. j Fluorescence of Cx3cr1 and Lp-PLA2 between SiglecFloAMs and SiglecFhiAMs. The data are presented as the means ± SDs; *p < 0.05, **p < 0.01, ***p < 0.001, ns p ≥ 0.05
