Fig. 3: Characterization of primary ILC2 cultures. | Cellular & Molecular Immunology

Fig. 3: Characterization of primary ILC2 cultures.

From: Macrophages regulate PD-1 and CTLA-4 expression on ILC2s and their responsiveness in the tumor microenvironment

Fig. 3

A Expression of CRTH2, KLRG1, CD7, CD161 and LIN (CD3, CD14, CD19, CD20, CD56, and CD16) on cultured ILC2s; B expression of CD28 in cultured ILC2s and T cells (gated as CD3+ among freshly isolated HD PBMCs); and C expression of GATA3 and T-BET in cultured ILC2s and cultured NK cells. D Intracellular IL-13 and IFN-γ staining of cultured ILC2 and NK cells unstimulated or stimulated with PMA/ionomycin. E Expression of CD45RA and CD45RO on freshly isolated ILC2s and cultured ILC2s. F PD-1 and CTLA-4 expression on cultured ILC2s. A, B, F FMOs are shown as negative controls. FACS plots are shown for one representative HD. G Percentages of dead THP-1 target cells (identified as Annexin V+/7-AAD+) after 48 h of coculture with ILC2s or NK cells at the indicated effector:target (E:T) ratios. The data are shown as the means +/−SDs of 3 independent experiments for ILC2s and 1 representative experiment for NK cells

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