Fig. 4: Macrophages inhibit ILC2 effector functions. | Cellular & Molecular Immunology

Fig. 4: Macrophages inhibit ILC2 effector functions.

From: Macrophages regulate PD-1 and CTLA-4 expression on ILC2s and their responsiveness in the tumor microenvironment

Fig. 4

A RNA sequencing was performed to compare gene expression between ILC2s sorted from coculture with MoMs and ILC2s cultured alone. DEGs between each pair of samples were identified from the normalized gene-level counts via the following cutoff values: fold change of 2 and adjusted P value ≤ 0.05. Pathway enrichment analysis was performed with Panther on the set of DEGs between ILC2s cocultured with MoMs and ILC2s cultured alone. The heatmap shows normalized counts for the DEGs belonging to the “Interleukin signaling” pathway in the 2 conditions (n = 3 donors). B IL5, IL4 and IL13 gene expression normalized to that of 18S was confirmed by RT‒PCR. The histograms show the fold change relative to ILC2s. C IL-5, IL-4 and IL-13 cytokine concentrations were also analyzed in the supernatant after coculture by bead-based multiplex flow cytometry. N.D. not detected. The histograms show the means +/− SDs of 4 (B) and 5 (C) independent experiments. Ratio paired t tests between 2−ΔCt values (B) and t tests (C) were performed. *p < 0.05, **p < 0.01, ***p < 0.005

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