Fig. 1: Accumulation of CD8⁺ T cells within the hematoma during the hyperacute phase of ICH.

A Experimental design for single-cell sequencing and flow cytometry of immune cells from ICH patients and controls. Created with BioRender.com. B CT images of 4 ICH patients and quantification of PHE volume (in red) and ICH volume (in green). C UMAP plot of 79,721 cells derived from mononuclear cells of the peripheral blood and hematoma. Bar plots showing the proportions of different cell types in the indicated groups of subjects. D UMAP plot showing the cell density in the indicated groups of subjects. E Proportions of CD8⁺ T cells and B cells identified from the scRNA-seq data of the indicated groups of subjects. ICH patients: n = 4; controls: n = 4. F Flow cytometry plots showing the gating strategy of T cells for mononuclear cells in the peripheral blood and hematoma. G Bar graphs showing the proportions of B cells and T cells among total lymphocytes and the proportions of CD4⁺ T cells and CD8⁺ T cells among T cells in the indicated groups of subjects. ICH patients: n = 10; controls: n = 10. Mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. PB (Control): peripheral blood of controls; PB (ICH): peripheral blood of ICH patients; Hematoma (ICH): Hematoma blood of ICH patients