Fig. 1: Lipid accumulation in mature low-density neutrophils in the blood increases in advanced colorectal cancer. | Cellular & Molecular Immunology

Fig. 1: Lipid accumulation in mature low-density neutrophils in the blood increases in advanced colorectal cancer.

From: IL33-induced lipid droplet formation in mature low-density neutrophils drives colorectal cancer liver metastasis

Fig. 1

A Lipid staining of neutrophils from colorectal cancer patients detected by BODIPY 493/503 and Oil Red O. Lipid quantification by flow cytometry (n = 35). Scale bar, 5 μm. B Intracellular lipids of patient neutrophils in different tumor stages quantified by flow cytometry (n = 8, 9, 11 and 7 for stages I to IV, respectively). MFI, mean fluorescence intensity. C, D Lipid levels in neutrophils from the peripheral blood and ascites of different tumor-bearing BALB/c mice (n = 3). T.F., tumor-free. S.C., subcutaneous. L.M., liver metastasis. B.A., bloody ascites. E Relative mRNA expression of genes related to lipid uptake and synthesis in M-LDNs (n = 3). F Lipid content quantification of neutrophils in the peripheral blood and liver of tumor-bearing mice (n = 3). G, H Lipid levels in liver-infiltrating neutrophils from different tumor-bearing BALB/c mice quantified by BODIPY 493/503 and TG levels (n = 3). I Proportion of Ly6G+ liver-infiltrating neutrophils in BALB/c mice at different stages (n = 3)

Back to article page