Fig. 1
From: AIM2 drives inflammatory cell death and monkeypox pathogenesis
Identification of AIM2 as a cytosolic innate immune sensor for monkeypox virus. A Heatmap illustrating serum cytokine/chemokine concentration levels in patients with monkeypox infection. The values for monkeypox virus (MPXV) patients were acquired from previously published cytokine assays [2], which categorize patients on the basis of disease severity. Normal values were sourced from the Bio-Plex® suspension array system tech note 6029 in Bio-Rad. The fold change was calculated for each disease severity compared with normal values as the baseline, represented on a logarithmic scale. Cytokines were ordered by the fold change under “severe” conditions. B–D Assessment of IL-1β release (B), cell death (C), and lactate dehydrogenase (LDH) release (D) in wild-type (WT) or the indicated cytosolic innate immune sensor-deficient immortalized bone marrow-derived macrophages (iBMDMs) following MPXV infection (MOI of 0.1 for 24 h). B and D Data are presented as the means ± s.e.m. ****P < 0.0001 (one-way ANOVA with Dunnett’s multiple comparisons test; n = 9 from 3 biologically independent samples). (C) shows images representative of a minimum of three independent experiments. Scale bar: 50 μm
