Fig. 6: Loss of SPT6 disrupts keratinocyte state transitions and promotes inflammatory transcription factor regulons and pathway signatures in specific subclusters. | Cellular & Molecular Immunology

Fig. 6: Loss of SPT6 disrupts keratinocyte state transitions and promotes inflammatory transcription factor regulons and pathway signatures in specific subclusters.

From: SPT6 maintains epidermal homeostasis by inhibiting an NF-κB-positive feedback loop to prevent excessive inflammation

Fig. 6: Loss of SPT6 disrupts keratinocyte state transitions and promotes inflammatory transcription factor regulons and pathway signatures in specific subclusters.

CellPhoneDB analysis of keratinocyte subcluster interactions in control (A) and Supt6-KO (B) mouse epidermis. The heatmap displays the predicted strength of ligand–receptor–mediated communication between keratinocyte subclusters. The color intensity represents the interaction strength, with red indicating the strongest predicted interactions. Ranked regulon-specific score (RSS) from SCENIC analysis in keratinocyte subclusters 5 (5-Bas-2) (C) and 4 (4-Interm-3) (D). E SCENIC heatmap of transcription factor activity in keratinocyte subclusters 4 and 5. The color key from blue to red indicates low to high expression levels. The number in parentheses represents the number of genes in the regulon. F Regulon activity in control (teal) and Supt6-KO (red) epidermis across corresponding keratinocyte subclusters. Box plots showing the expression distributions of the Cebpd, Jund, Atf3, Lhx2, Rel, and Klf4 regulons. G GSEA of genes enriched in keratinocyte subcluster 5 (5-Bas-2). Dot plot summarizing the top activated and suppressed pathways enriched in 5-Bas-2, with the NES on the x-axis. Dot size indicates the number of genes enriched in each pathway, while color reflects statistical significance (adjusted p value), with red indicating greater significance. H GSEA of genes enriched in subcluster 4 (4-Interm-3). The dot plot displays the top activated and suppressed pathways enriched in 4-Interm-3, with the NES on the x-axis. Dot size indicates the number of genes enriched in each pathway, while color reflects statistical significance (adjusted p value), with red indicating greater significance. Statistical significance is indicated as follows: *p < 0.05, **p < 0.01, and ****p < 0.0001 (t-tests were performed for comparisons between two groups)

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