Fig. 8: SPT6 inhibits the positive feedback transcriptional activation of RELA through binding to its enhancer region.

A RELA mRNA expression in control and SPT6i cells treated with poly(I: C) was measured by RT‒qPCR. B, C Western blot analysis and quantification of SPT6, p-p65, and p65 expression in control and SPT6i cells treated with poly(I: C). D Gene track of the RELA gene. The SPT6 ChIP-Seq signal and peaks are shown in dark blue. H3K27ac ChIP-Seq signals and peaks are shown in red. H3K4me1 ChIP-Seq signals and peaks are shown in green. The p65 ChIP-Seq signal and peaks are shown in gray. The y-axis shows the number of reads per million, and the colored bars above the signal tracks denote peak calls. The p65 binding motif predicted by JASPAR (motif ID: MA0107.1; relative score: 0.800986) is displayed at the bottom of the track. E ChIP‒qPCR of p65 binding at the RELA enhancer and promoter region in control and SPT6i cells treated with poly(I: C). F Schematic diagram of the proposed mechanism. The mean values are shown with error bars representing the SDs. Each dot in the graph represents a single replicate or data from an individual sample. Statistical significance is indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s multiple comparison for 3 groups, and a t-test was performed for comparisons between two groups). n ≥ 3 per group