Fig. 7
From: Construction of a 3D mammary duct based on spatial localization of the extracellular matrix
Permeability of 3D mammary epithelium under various ECM. a Schematic illustration of mammary duct permeability measurement (left). FITC-dextran (10 μM) in cell culture medium was introduced into the mammary duct channel (center), and the fluorescence intensity was monitored 3 h later (right). Inset shows FITC intensity profile from the mammary duct channel into the COL1 hydrogel (white arrow). Scale bar, 200 µm. b Comparison of blockage/leakage ratio (left) and relative permeability value (right) under various coating conditions. White and black bars indicate leakage and blockage ratios, respectively. Relative permeability values (means ± standard errors) are compared with those under optimal conditions (COL1-coated (200 µg/ml) COL1 hydrogel) (**P < 0.01 and ***P < 0.001, n = 29). HD indicates high density, and LN-c, MAT-c, and CO1-c indicate LN-, MAT-, and COL1-coated, respectively
