Fig. 2: Rheological and biological characterization of hydrogels.

a Small amplitude oscillatory shear tests: storage modulus (G′) and loss modulus (G″) as a function of frequency for COLL, COLL-LMW HA, and COLL-HMW HA gels, 3 replicates/group. b Steady shear tests: viscosity as a function of shear rate for COLL, COLL-LMW HA, and COLL-HMW HA gels, 3 replicates/group. c Injectability tests: typical load-displacement curves for gel-based systems injected through a 30G needle (solid line) or an empty syringe (dotted line). d SH-SY5Y cell metabolic activity with time after embedding in COLL, COLL-LMW HA, and COLL-HMW HA gels. The results from MTS assay, 6 replicates/group. We analyzed the results with two-way ANOVA followed by Bonferroni's multiple comparisons test. ns, p > 0.05; *p < 0.05; ***p < 0.001. Results are presented as mean ± SD