Fig. 5: MTT assay results of EMNPs in two cell lines and HE staining of rat tissue slices.

The MTT results of two cell lines, HUVEC and RAW 264.7, showed good biocompatibility of EMNPs at different concentrations (a, b). HE staining of rat tissue slices of the heart, liver, spleen, lung, kidney and cerebrum in groups B (high-fat diet) and F (high EMNP shaking), which presented no inflammation or atypia and definitely normal morphological characteristics (c). HE staining microscopy images under 200× magnification. (d, e) Average proximal intestine villi/crypt ratio (d) and distal intestine villi/crypt ratio (e). f Hemolysis ratio of EMNPs. Triton X-100 was set as a control