Fig. 3: Biological studies of Schwann cells (SCs) and RAW264.7 cells on nanodiamond/polycaprolactone (ND/PCL) and PCL scaffolds in vitro. | NPG Asia Materials

Fig. 3: Biological studies of Schwann cells (SCs) and RAW264.7 cells on nanodiamond/polycaprolactone (ND/PCL) and PCL scaffolds in vitro.

From: Multilayered spraying and gradient dotting of nanodiamond–polycaprolactone guidance channels for restoration of immune homeostasis

Fig. 3

Western blot and quantitative polymerase chain reaction (qPCR) assays for proliferation and neural expression using Ki67, S100, Tuj1, and myelin basic protein (MBP) in SCs between ND/PCL and PCL scaffolds. Blots of different markers in two scaffolds (a). Relative Ki67 mRNA levels (b). Relative S100 mRNA levels (c). Relative Tuj1 mRNA level (d). Relative MBP mRNA levels (e). *p < 0.05 compared with PCL. SC attachment evaluation using scanning electron microscopy between two scaffolds. The images showed different SC protuberances in ND/PCL (f–h) and PCL (i–k) scaffolds. Scale bars: 100 μm (f, i); 50 μm (g, j); 20 μm (h, k). Western blot and qPCR assays for macrophage polarization using interleukin (IL)-6, tumor necrosis factor (TNF)-α, CD206, and IL-10 in RAW264.7 cells between ND/PCL and PCL scaffolds. Blots of different markers in two scaffolds (l). Relative IL-6 mRNA levels (m). Relative TNF-α mRNA level (n). Relative CD206 mRNA level (o). Relative IL-10 mRNA levels (p). Relative signal transducer and activator of transcription-3 level (q). Relative nuclear factor-κB levels (r). *p < 0.05 compared with PCL

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