Fig. 7: Immunofluorescence assay of regenerated sciatic nerves using Tuj1 and NF200 for axon regeneration assessment at 4 months postoperatively.

Immunofluorescence assay of regenerated sciatic nerves using Tuj1 (a–i) and NF200 (j–r) for axon regeneration assessment at 4 months postoperatively. Nanodiamond/polycaprolactone (PCL) nerve bridge (a–c, j–l). PCL nerve bridge (d–f, m–o). Autograft (g–i, p–r). Nuclear staining (a, d, g, j, m, p). Marker staining (b, e, h, k, n, q). Merged images (c, f, i, l, o, r). Scale bar = 100 μm. Relative Tuj1 level (s). Relative NF200 level (t). *p < 0.05 compared with autograft, #p < 0.05 compared with PCL