Fig. 11: Measurement of the network mesh size and visualization of the compartmentalized architecture.

a Confocal imaging of Brownian motions of beads in solution and hydrogel matrix after addition of BAP. The right, middle, and left micrographs show nanofibers, fluorescent beads, and merged images, respectively. b Red (left) and green (middle) CLSM images show the positions of the fluorescence beads at 0 and 5 min, while the yellow points in merged images represent the immobile beads. The numbers indicate the self-sorting degree quantified via Pearson’s correlation coefficient (r). c Brownian motions of beads with different diameters in solution and hydrogel matrix. a–c Reproduced with permission⁵⁸. Copyright 2018, Springer Nature. d Schematic illustration of the incorporation of fluorophores in liposomes and self-assembled fibers. e Compartmentalized architecture visualized by fluorescence lifetime imaging microscopy based on lifetime discrepancies. d, e Reproduced with permission⁵⁹. Copyright 2016, Royal Society of Chemistry.