Fig. 7: Encapsulation of exosomes in hydrogels and the release of exosomes from hydrogels through physical/chemical interactions.

A ADSC-derived exosomes were loaded in an FEP hydrogel through electrostatic interactions and released in response to pH changes. (a) Schematic illustration of the synthesis of a multifunctional FEP hydrogel scaffold. (b) Schematic of exosome release from the FEP scaffold dressing. (c) Representative pH-dependent release profile of loaded exosomes in the FHE scaffold dressing. Reproduced with permission¹⁰⁴. Copyright 2019, American Chemical Society. B Light-triggerable HA hydrogel for the controlled release of SEVs. (a) Schematic illustration of the formation of the light-triggerable HA hydrogel containing SEVs (hydrogel is translucid: a1, swollen gel in PBS; a2, swollen gel in DMEM). Photocleavage of the HA hydrogel over time demonstrating that the release of SEVs was dependent on (b) the number of irradiations and (c) the exposure time. *p < 0.05, **p < 0.01, ***p < 0.001, UV-light group vs. nonirradiated group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, blue-light group vs. nonirradiated group. Reproduced with permission¹⁰⁵. Copyright 2019, American Chemical Society.