Fig. 3: In vitro biocompatibility, cell attachment, and osteogenic expression evaluation.
From: Tough and biodegradable polyurethane-silica hybrids with a rapid sol-gel transition for bone repair
A CCK-8 assay performed in accordance with ISO 10993. MC3T3-E1 cells were cultured with the dissolution products of BPHS. Polyethylene (PE) media conditioned with a nontoxic substance was used as the negative control, and polyurethane (PU) was used as the positive control. B Confocal microscopy images of F-actin and nuclei stained MC3T3-E1 (F-actin labeling in red, and DAPI nuclear counterstain in blue) for (i) BPHS0, (ii) BPHS10, (iii) BPHS20, and (iv) BPHS30 (Scale bar: 200 μm). C Accelerated degradation test conducted on BPHS. Change in the mass of BPHS after the accelerated degradation test (n = 4) (*p < 0.05 and **p < 0.01). BPHS was immersed in 1 M NaOH at 25 °C for 0, 8, 24, 48, 72, and 168 h. D Gene expression of osteogenic differentiation markers (n = 3): (i) RUNX2, (ii) ALPL, and (iii) COL1A1 (*p < 0.05 and **p < 0.01). E Silica dissolution profile of BPHS30 upon immersion in PBS solution over 1, 2, and 4 weeks (n = 3).
