Fig. 1: A high-throughput microfluidic device for reproducing three-dimensional lymphangiogenesis in vitro. | NPG Asia Materials

Fig. 1: A high-throughput microfluidic device for reproducing three-dimensional lymphangiogenesis in vitro.

From: An advanced 3D lymphatic system for assaying human cutaneous lymphangiogenesis in a microfluidic platform

Fig. 1

a Schematic design of each chip of the high-throughput device. The channels painted blue are media channels, and pink channels are gel channels. Media channels were filled with media containing cytokines and cells. Gel channels were filled with each concentration of collagen type I. b Top view of the whole 24-well PDMS high-throughput microfluidic device. This device included 24 lymphangiogenesis chips. c Experimental flow diagram of lymphangiogenesis modeling. Each gel channel was filled with seeding cells. The HDLECs were seeded in channel #5 and cultured for three days. After the formation of the HDLEC single layer, the cells were treated with Lymphanax for six days. d Immunofluorescence images of the HDLEC single layer on Day 3. The image above is a top view of the HDLEC single layer, and the image below is a side view of the HDLEC single layer. Prox1 (green) is expressed in lymphatic cells and HDLECs. The localization of HDLEC was indicated by F-actin (Red). Scale bars indicate 200 µm.

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