Fig. 6: The cutaneous lymphatic system model was completed by adding the functional cutaneous barrier to the lymphangiogenesis model. | NPG Asia Materials

Fig. 6: The cutaneous lymphatic system model was completed by adding the functional cutaneous barrier to the lymphangiogenesis model.

From: An advanced 3D lymphatic system for assaying human cutaneous lymphangiogenesis in a microfluidic platform

Fig. 6

a Experimental flow diagram of creating the cutaneous lymphatic system model. Each gel channel was filled before a day seeding cells. NHEKs were cultured in channel #1, and HDLECs were cultured in channel #5. b Immunofluorescence images of the differentiated cutaneous barrier (two upper panels) and lymphatic single layers (two lower panels) in the cutaneous lymphatic system model. c Images showing the permeability test for the functional skin barrier by 40 kDa FITC-dextran (green). d Graph represents the results of the permeability test by each size of substance. Scale bars indicate 200 µm.

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