Fig. 4: In vitro osteogenic differentiation-modulating property of the GMOS-based hydrogel.
From: A bone adhesive enhances osteoporotic fracture repair by regulating bone homeostasis
A ALP staining of BMSCs cultured on each adhesive for 7 days (scale bar: 100 μm). B ARS staining of BMSCs cultured on each adhesive for 14 days (scale bar: 100 μm). C Quantitative analysis of ALP staining (n = 3). D Quantitative analysis of ARS staining (n = 3). E, F The expression of osteogenesis-related genes, including OPN, RunX2, OCN, and Col-1, was estimated by RT‒qPCR after BMSCs were cultured on the hydrogel surface for 14 days (n = 3). G Immunofluorescence images showing OPN and RunX2 staining of BMSCs cultured on the hydrogel surface for 14 days (scale bar: 50 μm). OPN and RunX2 were stained red, and the nuclei were stained blue. H Quantitative analysis of immunofluorescence staining (n = 3). I The protein expression of RunX2 and OPN was evaluated by Western blotting. J Quantitative analysis of the Western blotting data (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001.
