Abstract
Double helical polysaccharide, xanthan samples with varying molar mass were thermally denatured and renatured in dilute solutions. Both the weight average molar mass, which was determined by size exclusion chromatography (SEC), and viscosity average molar mass decreased after denaturation and renaturation for samples with an initial molar mass of 106 g mol−1, but those for the samples with an initial molar mass of 105 or 107 g mol−1 decreased only slightly. Although the double helices of xanthan only partially dissociated into single chains, the molar mass distribution estimated by SEC did not broaden drastically by the denaturation and renaturation. These results can be explained by the formation of hairpin structures by the single chains, which are restricted to xanthan with a molar mass of 105 g mol−1 by the strain of the hairpin loop, and for xanthan molar masses of 107 g mol−1 by the incomplete dissociation of its long polymer chains.
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References
Norisuye T, Teramoto A. Polymeric materials encyclopedia. In: Salamone JC, editor. Boca Ratonp, RC Press; 1996. p. 8801–9.
Sato T, Norisuye T, Fujita H. Double-stranded helix of xanthan in dilute solution: evidence from light scattering. Polym J. 1984;16:341–50.
Sato T, Kojima S, Norisuye T, Fujita H. Double-stranded helix of xanthan in dilute solution: further evidence. Polym J. 1984;16:423–9.
Sato T, Norisuye T, Fujita H. Double-stranded helix of xanthan in dilute solution: dimensional and hydrodynamic properties in 0.1 M aqueous sodium chloride. Macromolecules. 1984;17:2696–700.
Liu W, Sato T, Norisuye T, Fujita H. Thermally induced conformational change of xanthan in 0.01 M aqueous sodium chloride. Carbohyd Res. 1987;160:267–81.
Liu W, Norisuye T. Order-disorder conformation change of xanthan in 0.01 m aqueous sodium chloride: dimensional behavior. Biopolymers. 1988;27:1641–54.
Capron G, Brigand G, Muller G. About the native and renatured conformation of xanthan exopolysaccharide. Polymer. 1997;38:5289–95.
Oviatt HW Jr., Brant DA. Viscoelastic behavior of thermally treated aqueous xanthan solutions in the semidilute concentration regime. Macromolecules. 1994;27:2402–8.
Matsuda Y, Biyajima Y, Sato T. Thermal denaturation, renaturation, and aggregation of a double-helical polysaccharide xanthan in aqueous solutions. Polym J. 2009;41:526–32.
Matsuda Y, Sugiura F, Mays JW, Tasaka S. Atomic force microscopy of thermally renatured xanthan with low molar mass. Polym J. 2015;47:282–5.
Mohri K, Nishikawa M, Takahashi N, Shiomi T, Matsuoka N, Ogawa K, Endo M, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y. Design and development of nanosized DNA assemblies in polypod-like structure as efficient vehicles for immunostimulatory CpG motifs to immune cells. ACS Nano. 2012;6:5931–40.
Osada K, Oshima H, Kobayashi D, Doi M, Enoki M, Yamasaki Y, Kataoka K. Quantized folding of plasmid DNA condensed with block catiomer into characteristic rod structure promoting transgene efficacy. J Am Chem Soc. 2010;132:12343–8.
Choppe E, Puaud F, Nicolai T, Benyahia L. Rheology of xanthan solutions as a function of temperature, concentration and ionic strength. Carbohyd Polym. 2010;82:1228–35.
Papagiannopoulos A, Sotiropoulos K, Pispas S. Particle tracking microrheology of the power-law viscoelasticity of xanthan solutions. Food Hydrocoll. 2016;61:201–10.
Ikeda S, Gohtani S, Nishinari K, Zhong Q. Single molecules and networks of xanthan gum probed by atomic force microscopy. Food Sci Technol Res. 2012;18:741–5.
Moffat J, Morris VJ, Al-Assaf S, Gunning AP. Visualisation of xanthan conformation by atomic force microscopy. Carbohyd Polym. 2016;148:380–9.
Matsuda Y, Sugiura F, Okumura K, Tasaka S. Renaturation behavior of xanthan with high molar mass and wide molar mass distribution. Polym J. 2016;48:653–8.
García-Ochoa F, Santos VE, Casas JA, Gómez E. Xanthan gum: production, recovery, and properties. Biotechnol Adv. 2000;18:549–79.
Acknowledgements
We thank Prof. Takahiro Sato at Department of Macromolecular Science, Osaka University who kindly provided the xanthan samples, allowed us to use the CD instrument, and advised us about the stability of the hairpin structure. This work is partially supported by grants from The Japan Health Foundation and Mishima Kaiun Memorial Foundation.
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Matsuda, Y., Okumura, K. & Tasaka, S. Molar mass dependence of structure of xanthan thermally denatured and renatured in dilute solution. Polym J 50, 1043–1049 (2018). https://doi.org/10.1038/s41428-018-0098-7
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DOI: https://doi.org/10.1038/s41428-018-0098-7
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